ABSTRACT
Objective To investigate the band pattern characteristics of Western bolt (WB) by analyzingthe change of anti-virus antibodies from patients with different clinical stages of human immunodeficiency virus 1 (HIV-1) infection.Methods Antibodies were detected by WB test,CD4+ T cells by flow cytometer.Results Among 208 cases,193 male cases accounted for 92.79％,and 163 cases of men who have sex with men (MSM) accounted for 78.37％.The antibodies against viral protein gp160,gp120,gp41,p66,p51,p31,p24 showed high positive rates,and no significant different was detected across different clinical stages.The positive rate of p55 antibody,encoding by gag gene,was significantly higher in those HIV positive individuals infected via homosexual compared with HIV-1 positive individuals through heterosexual contact (P＜0.05).The 5 common WB bands patterns were as following:all bands①,missing p55②,missing p39③,missing p55 + p39④ and missing p39 + p55 + p17⑤.Primary stage infection group showed the highest appearance rate of all bands (44.2％).Conclusions The WB bands patterns and CD4+ T lymphocyte counts can help to judge immune status,determine disease stages and monitor disease progression.We should take effective measures for the MSM and floating population.
ABSTRACT
Purpose To summarize the clinicopathologic features,diagnosis and differential diagnosis of nodular fasciitis (NF) of the breast.Methods Clinicopathologic findings of four mammary NF cases were retrospectively reviewed.Immunohistochemical of EnVision staining was performed in all cases.Results The median age of all patients was 50.5 years.All patients were female,without history of trauma.The average size was 1.2 cm.Among the four cases reviewed,three lesions occurred in the mammary parenchyma and one next to the skin.Histologically,the lesion was composed of plump spindle cells arranged in short fascicles with erythrocytes extravasation and patchy lymphoid infiltration,and the border of three cases was infiltrative.Immunohistochemically,the spindle cells were positive for vimentin,CD10 and SMA,but negative for CK (AE1/AE3),CK5/6,CK14,CAM5.2,ER and PR.All patients underwent surgical resection,with no evidence of recurrence.Conclusion NF of the breast is rare.It should be differentiated from some benign and malignant tumours,such as fibromatosis,phyllodes tumor and low-grade fibromatosis-like metaplastic carcinoma.The immunostaining of vimentin and SMA may helpful for the diagnosis and differential diagnosis of NF of breast.
ABSTRACT
Objective To investigate the band pattern characteristics of Western bolt (WB) by analyzingthe change of anti-virus antibodies from patients with different clinical stages of human immunodeficiency virus 1 (HIV-1) infection.Methods Antibodies were detected by WB test,CD4+ T cells by flow cytometer.Results Among 208 cases,193 male cases accounted for 92.79％,and 163 cases of men who have sex with men (MSM) accounted for 78.37％.The antibodies against viral protein gp160,gp120,gp41,p66,p51,p31,p24 showed high positive rates,and no significant different was detected across different clinical stages.The positive rate of p55 antibody,encoding by gag gene,was significantly higher in those HIV positive individuals infected via homosexual compared with HIV-1 positive individuals through heterosexual contact (P＜0.05).The 5 common WB bands patterns were as following:all bands①,missing p55②,missing p39③,missing p55 + p39④ and missing p39 + p55 + p17⑤.Primary stage infection group showed the highest appearance rate of all bands (44.2％).Conclusions The WB bands patterns and CD4+ T lymphocyte counts can help to judge immune status,determine disease stages and monitor disease progression.We should take effective measures for the MSM and floating population.